The extant manuscripts of the book Antiquities of the Jews, written by the first-century Jewish historian Flavius Josephus around AD 93–94, contain two references to Jesus of Nazareth and one reference to John the Baptist.

The first and most extensive reference to Jesus in the Antiquities, found in Book 18, states that Jesus was the Messiah and a wise teacher who was crucified by Pontius Pilate. It is commonly called the Testimonium Flavianum. Almost all modern scholars reject the authenticity of this passage in its present form, while most scholars nevertheless hold that it contains an authentic nucleus referencing the life and execution of Jesus by Pilate, which was then subject to Christian interpolation or alteration. However, the exact nature and extent of the Christian addition remains unclear.

Modern scholarship has largely acknowledged the authenticity of the second reference to Jesus in the Antiquities, found in Book 20, Chapter 9, which mentions "the brother of Jesus, who was called Christ, whose name was James." This reference is considered to be more authentic than the Testimonium.

Almost all modern scholars consider the reference in Book 18, Chapter 5 of the Antiquities to the imprisonment and death of John the Baptist also to be authentic and not a Christian interpolation. A number of differences exist between the statements by Josephus regarding the death of John the Baptist and the New Testament accounts. Scholars generally view these variations as indications that the Josephus passages are not interpolations, since a Christian interpolator would likely have made them correspond to the New Testament accounts, not differ from them. Scholars have provided explanations for their inclusion in Josephus' later works.